abstract：:Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...

journal_title：Cell structure and function

authors： Matsumura F,Totsukawa G,Yamakita Y,Yamashiro S

更新日期：2001-12-01 00:00:00

abstract：:In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...

journal_title：Cell structure and function

authors： Kusano I,Kageyama A,Tamura T,Oda T,Muramatsu T

更新日期：2001-10-01 00:00:00

abstract：:It was recently reported that intravenous administration of phorbol 12-myristate 13-acetate (PMA) showed a therapeutic effect in myelocytic leukemia patients. However, we previously observed that, in serum-free conditions, polymorphonuclear leucocytes (PMNs) were killed rapidly by exposure to PMA, suggesting the possi...

journal_title：Cell structure and function

authors： Suzuki K,Kato H,Sakuma Y,Namiki H

更新日期：2001-08-01 00:00:00

abstract：:Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...

journal_title：Cell structure and function

authors： Ghert MA,Qi WN,Erickson HP,Block JA,Scully SP

更新日期：2001-06-01 00:00:00

abstract：:The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...

journal_title：Cell structure and function

authors： Sato Y

更新日期：2001-02-01 00:00:00

abstract：:CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

journal_title：Cell structure and function

authors： Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

更新日期：2000-10-01 00:00:00

abstract：:Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...

journal_title：Cell structure and function

authors： Imamoto N

更新日期：2000-08-01 00:00:00

abstract：:Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

journal_title：Cell structure and function

authors： Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

更新日期：2000-06-01 00:00:00

abstract：:The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...

journal_title：Cell structure and function

authors： Russell L,Naora H,Naora H

更新日期：2000-04-01 00:00:00

abstract：:A large number of Rab/Ypt-family small GTPases have been identified from higher plants. While some of them can complement yeast ypt mutants, the expression of Arabidopsis Ara4 protein aggravated the growth defect of a subset of ypt mutants, probably because of the titration of common regulator(s) of yeast Ypt proteins...

journal_title：Cell structure and function

authors： Matsuda N,Ueda T,Sasaki Y,Nakano A

更新日期：2000-02-01 00:00:00

abstract：:Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...

journal_title：Cell structure and function

authors： Zong ZP,Fujikawa-Yamamoto K,Li AL,Yamaguchi N,Chang YG,Murakami M,Odashima S,Ishikawa Y

更新日期：1999-12-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Microtubules are intrinsically dynamic polymers. Two kinds of dynamic behaviors, dynamic instability and treadmilling, are important for microtubule function in cells. Both dynamic behaviors appear to be tightly regulated, but the cellular molecules and the mechanisms responsible for the regulation remain largely unex...

journal_title：Cell structure and function

authors： Wilson L,Panda D,Jordan MA

更新日期：1999-10-01 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

journal_title：Cell structure and function

authors： Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

更新日期：1999-04-01 00:00:00

abstract：:The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathi...

journal_title：Cell structure and function

authors： Kanaseki T,Ikeuchi Y,Tashiro Y

更新日期：1998-12-01 00:00:00

abstract：:Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...

journal_title：Cell structure and function

authors： Nishiyama R,Ikami M,Yamao F,Tsuchida S,Yagura T

更新日期：1998-10-01 00:00:00

abstract：:Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...

journal_title：Cell structure and function

authors： Sugiyama N,Tabuchi Y,Numata F,Uchida Y,Horiuchi T,Ishibashi K,Ono S,Obinata M,Furusawa M

更新日期：1998-06-01 00:00:00

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00

abstract：:A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

journal_title：Cell structure and function

authors： Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

更新日期：1997-08-01 00:00:00

abstract：:We used a morphological approach to determine the topogenic role of the signal peptide in mediating the ER translocation of yeast prepro-alpha-factor. In prepro-alpha-factor-somatostatin hybrids, changes in the N-terminal amino acid sequence from wild-type NH2-Met-Arg-Phe (MRF) to NH2-Met-Phe-Lys (MFK) caused a subtle...

journal_title：Cell structure and function

authors： Cheong KH,Park SD,Kim J,Hong SH

更新日期：1997-06-01 00:00:00

abstract：:alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...

journal_title：Cell structure and function

authors： Sudou A,Muramatsu H,Kaname T,Kadomatsu K,Muramatsu T

更新日期：1997-04-01 00:00:00

abstract：:Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...

journal_title：Cell structure and function

authors： Fulton AB,Alftine C

更新日期：1997-02-01 00:00:00

abstract：:We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...

journal_title：Cell structure and function

authors： Auerbach D,Rothen-Ruthishauser B,Bantle S,Leu M,Ehler E,Helfman D,Perriard JC

更新日期：1997-02-01 00:00:00

abstract：:Skeletal muscle contraction is regulated by a complex of five polypeptides which are stably associated with the actin filament. This complex consists of two proteins: troponin with three subunits (TnC; TnI and TnT) and tropomyosin (a dimer of two chains). Using deletion mutants of TnC, TnI and TnT we determined that e...

journal_title：Cell structure and function

authors： Reinach FC,Farah CS,Monteiro PB,Malnic B

更新日期：1997-02-01 00:00:00

abstract：:In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...

journal_title：Cell structure and function

authors： Miya F,Yamamoto A,Akagawa K,Kawamoto K,Tashiro Y

更新日期：1996-12-01 00:00:00

abstract：:The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...

journal_title：Cell structure and function

authors： Pollard TD,Ostap EM

更新日期：1996-10-01 00:00:00

abstract：:1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

journal_title：Cell structure and function

authors： Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

更新日期：1996-10-01 00:00:00

abstract：:Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...

journal_title：Cell structure and function

authors： da Silva NS,Dias Filho BP,De Souza W

更新日期：1996-08-01 00:00:00

abstract：:Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...

journal_title：Cell structure and function

authors： Mihara K,Iijima M,Kondo T,Namba M

更新日期：1996-04-01 00:00:00

abstract：:To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

journal_title：Cell structure and function

authors： Hase H,Hirayama E,Kim J

更新日期：1996-02-01 00:00:00

abstract：:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...

journal_title：Cell structure and function

authors： Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H

更新日期：1995-10-01 00:00:00

abstract：:The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

journal_title：Cell structure and function

authors： Ohta T,Matsuda M

更新日期：1995-08-01 00:00:00

abstract：:Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...

journal_title：Cell structure and function

authors： Kato S,Sugiura N,Kimata K,Kujiraoka T,Toyoda J,Akamatsu N

更新日期：1995-06-01 00:00:00

abstract：:To construct recombinant retroviruses with only a single active promoter, we introduced point mutations into the TATA box region of the 3'-LTR, and successfully obtained high-titer virus with sufficient self-inactivating activity. However, the viral titer could not be determined by the number of G418 resistant colonie...

journal_title：Cell structure and function

authors： Miyao Y,Shimizu K,Tamura M,Yamada M,Tamura K,Nakahira K,Kuriyama S,Hayakawa T,Ikenaka K

更新日期：1995-04-01 00:00:00

abstract：:Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...

journal_title：Cell structure and function

authors： Akita N,Hoshi M

更新日期：1995-02-01 00:00:00

abstract：:The epidermal growth factor (EGF)-induced endocytosis of its receptor is an obligatory pathway for the cellular regulation of the EGF-specific receptor (EGF-R). BNER4 is a mouse Balb/3T3 cell line transfected with human EGF-R complementary DNA (cDNA). B4/src-13 and B4/src-24 are BNER4 cells transfected with a viral on...

journal_title：Cell structure and function

authors： Nakayama Y,Hisano T,Okimoto T,Tanaka Y,Ishikawa T,Himeno M,Ono M,Kuwano M

更新日期：1994-12-01 00:00:00

abstract：:The role of microtubule associated proteins (MAPs) on the dynamic instability of microtubules was examined under a dark-field microscope using bovine brain tubulin purified by DEAE-Sepharose column chromatography. In the absence of MAPs, the transition from the shortening phase to the growing phase (the rescue) occurr...

journal_title：Cell structure and function

authors： Itoh TJ,Hotani H

更新日期：1994-10-01 00:00:00

abstract：:When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...

journal_title：Cell structure and function

authors： Sameshima M,Chijiiwa Y,Kishi Y,Hashimoto Y

更新日期：1994-08-01 00:00:00